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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Opto-Injection Into Single Living Cells by Femtosecond NIR Laser

Opto-Injection Into Single Living Cells by Femtosecond NIR Laser

Peng, Cheng

Paperback

Physics

ISBN10: 3639098927
ISBN13: 9783639098921
Publisher: Blues Kids Of Amer
Published: Nov 25 2008
Pages: 88
Weight: 0.28
Height: 0.18 Width: 6.00 Depth: 9.00
Language: English
This book presents a novel technique of delivering membrane im-permeable molecules into single living cells with the assistance of femtosecond (fs) near-infrared (NIR) laser pulses. This technique promises several major advantages, namely, very high transfection efficiency, high cell survival rate (≈100%) and fully preserved cell viabilities. In this work, the system for fs NIR opto-injection was designed and built. Successful fs NIR opto-injection has been performed on several cell systems including single mammalian cells, marine animal eggs, and human cancer cells cultured in a tissue-like environment. The connections between laser parameters and cell responses were explored. Dye uptake rate of the target cells was observed to depend on incident laser intensity. Pore size was found dependent on incident laser intensity. The conclusion was made that laser-induced breakdown and plasma-induced ablation in cell membrane are the physical principles that govern the process of fs NIR opto-injection.

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