Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Oligonucleotide Synthesis: Methods and Applications

Oligonucleotide Synthesis: Methods and Applications

Herdewijn, Piet

Paperback

Series: Methods in Molecular Biology, Book 288

Medical ReferenceChemistry

ISBN10: 1617374415
ISBN13: 9781617374418
Publisher: Springer Nature
Published: Nov 10 2010
Pages: 436
Weight: 1.32
Height: 0.91 Width: 6.00 Depth: 9.00
Language: English
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

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