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Oligonucleotide Synthesis: Methods and Applications

Oligonucleotide Synthesis: Methods and Applications

Hardcover

Series: Methods in Molecular Biology, Book 288

Medical ReferenceChemistry

ISBN10: 1588292339
ISBN13: 9781588292339
Publisher: Springer Nature
Published: Sep 1 2004
Pages: 436
Weight: 1.87
Height: 1.17 Width: 14.36 Depth: 9.14
Language: English
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

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