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Nucleic Acid Detection: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1039
Medical ReferenceBiologyChemistry
ISBN13: 9781493960415
Publisher: Humana
Published: Aug 23 2016
Pages: 320
Weight: 1.29
Height: 0.70 Width: 7.00 Depth: 10.00
Language: English
Part I: Advances in Sample Preparation
1. Getting Blood from a Stone Ultra-Sensitive Forensic DNA Profiling of Microscopic Bio-particles Recovered from 'Touch DNA' Evidence
Erin K. Hanson and Jack Ballantyne
2. Detecting MicroRNA in Human Cancer Tissues with Fluorescence in situ Hybridization
Zonggao Shi, Jeff J. Johnson, and M. Sharon Stack
Part II: New Real-Time and Instantaneous Assays
3. MN Azyme qPCR: A Superior Tool for Multiplex qPCR
Elisa Mokay and Alison V. Todd
4. A New, Multiplex, Quantitative Real-time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification
Fang Liang, Neetika Arora, Kang Liang Zhang, David Che Cheng Yeh, Richard Lai, Darnley Pearson, Graeme Barnett, David Whiley, Theo Sloots, Simon R. Corrie, and Ross T. Barnard
5. Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with Universal Molecular Beacon Reporter
Yulia V. Gerasimova, Jack Ballantyne, and Dmitry M. Kolpashchikov
6. SNP Analysis using a Molecular Beacon-based Operating Cooperatively (OC) Sensor
Evan M. Cornett and Dmitry M. Kolpashchikov
Part III: Isothermal Amplification Methods for Nucleic Acid Detection
7. Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification
Wanyuan Ao and Robert Jenison
8. Rapid Detection of Brucella spp. using Loop-mediated Isothermal Amplification (LAMP)
Shouyi Chen, Xunde Li, Juntao Li, and Edward R. Atwill
9. Loop-mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms
Yasuhito Sako, Agathe Nkouawa, Tetsuya Yanagida, and Akira Ito
10. Detection of Mutation by Allele-specific Loop-mediated Isothermal Amplification (AS-LAMP)
Hiroka Aonuma, Athanase Badolo, Kiyoshi Okado, and Hirotaka Kanuka
Part IV: Signal Amplification Approac
11. DNA Detection by Cascade Enzymatic Signal Amplification
Bingjie Zou, Yinjiao Ma and Guohua Zhou
Part V: Non-Fluorescent Detection Formats
12. Visual DNA Detection and SNP Genotyping using Asymmetric PCR and Split DNA Enzymes
Jia Ling Neo and Mahesh Uttamchandani
13. Detection of Single-stranded Nucleic Acids via Colorimetric Means, using G-quadruplex Probes
Shizuka Nakayama and Herman O. Sintim
14. Lateral Flow Biosensors for the Detection of Nucleic Acid
Lingwen Zeng, Puchang Lie, Zhiyuan Fang, Zhuo Xiao
15. Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a
Wireless Bipolar Electrode
Mei-Sheng Wu, Guang-sheng Qian, Jing-Juan Xu, and Hong-Yuan Chen
16. High-throughput Label-free Detection of DNA Hybridization and Mismatch Discrimination Using Interferometric Reflectance Imaging Sensor
Sunmin Ahn, David S. Freedman, Xirui Zhang, and M. Selim Ünlü1
Part VI: Advances in Fish
17. Graphene -PAMAM Dendrimer -Gold Nano Particle Composite for Electrochemical DNA Hybridization Detection
Kumarasamy Jayakumar , Rajendiran Rajesh, Venkataraman Dharuman, and Rangarajan Venkatesan
18. Application of PNA Openers for Fluorescence-based Detection of Bacterial DNA
Irina Smolina
19. DNA probes for FISH analysis of C-Negative Regions in Human Chromosomes
Evgeniy S. Morozkin, Tatyana V. Karamysheva, Pavel P. Laktionov, Valentin V. Vlassov, and Nikolay B. Rubtsov
Part VII: Detection of microRNA
20. Quantitative Analysis of MicroRNA in Blood Serum with Protein-Facilitated Affinity Capillary Electrophoresis
Nasrin Khan and Maxim V. Berezovski
21. High-Throughput Functional MicroRNA Profiling using Recombinant AAV-Based MicroRNA Sensor Ar
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