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Nucleic Acid Aptamers: Selection, Characterization, and Application
Hardcover
Series: Methods in Molecular Biology, Book 2570
Technology & EngineeringBiologyChemistry
ISBN13: 9781071626948
Publisher: Humana
Published: Sep 27 2022
Pages: 282
Weight: 1.61
Height: 0.69 Width: 7.00 Depth: 10.00
Language: English
Part I: Selection
1. Automated ssDNA SELEX Using Histidine Tagged Target Proteins
Stefan Breuers and Günter Mayer
2. Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection
Nicole Williams, Rutika Patel, and Prabodhika Mallikaratchy
3. Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX
Moujab Choukeife, Anna Jonczyk, and Günter Mayer
4. Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction
Magda Puchala, Marta Radzińska, Joanna Guzdek, Agnieszka Sok-Grochowska, Aleksandra Adamowicz-Skrzypkowska, Pawel Pięta, Przemyslaw Jurek, and Monika Czarnecka
5. RNA Capture-SELEX on Streptavidin Magnetic Beads
Leon Kraus and Beatrix Suess
6. The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE
Jan Hoinka and Teresa M. Przytycka
7. Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX
Frédéric Ducongé
Part II: Characterization
8. Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry
Sladjana Slavkovic and Philip E. Johnson
9. Determination of Aptamer Structure Using Circular Dichroism Spectroscopy
Yannick Kerler, Stephan Sass, Carsten Hille, and Marcus M. Menger
10. Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis
Wiebke Sabrowski, Walter F.M. Stöcklein, and Marcus M. Menger
11. Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance
Nico Dreymann, Anja Möller, and Marcus M. Menger
12. Probing of Fluorogenic RNA Aptamers via Supramolecular Förster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog
Christian Steinmetzger and Claudia Höbartner
13. Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT
Sebastian Strauss and Ralf Jungmann
Part III: Application
14. Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environments
Nako Nakatsuka
15. Aptasensor for Impedimetric Detection of Lysozyme
Ece Eksin, Huseyin Senturk, and Arzum Erdem
16. Biophysical Analysis of Small Molecule Binding to Viral RNA Structures
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