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Nitrogen Fixation: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 766
ISBN13: 9781617791932
Publisher: Humana
Published: Aug 17 2011
Pages: 326
Weight: 1.77
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
Part I: Nitrogen Fixation
1. Historic Overview of Nitrogenase Research
Yilin Hu and Markus W. Ribbe
2. Mechanism of Mo-Dependent Nitrogenase
Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt
3. Assembly of Nitrogenase MoFe Protein
Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, and Yilin Hu
4. Genomic Analysis of Nitrogen Fixation
Ina P. O'Carroll and Patricia C. Dos Santos
5. Enzymatic Systems with Homology to Nitrogenase
Markus J. Bröcker and Jürgen Moser
Part II: Genetic and Biochemical Methods
6. Molecular Biology and Genetic Engineering in Nitrogen Fixation
Patricia Dos Santos
7. Purification of Nitrogenase Proteins
Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Hu, and Markus W. Ribbe
8. Assays of Nitrogenase Reaction Products
William E. Newton and Michael J. Dilworth
9. Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase
Markus J. Bröcker and Jürgen Moser
Part III: Spectroscopic Methods
10. X-Ray Crystallography
Lauren E. Roth and F. Akif Tezcan
11. X-Ray Absorption Spectroscopy
Serena DeBeer
12. Small Angle X-Ray Scattering Spectroscopy
David W. Mulder and John W. Peters
13. Electron Paramagnetic Resonance Spectroscopy
Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt
14. Magnetic Circular Dichroism Spectroscopy
Brian J. Hales
15. Mössbauer Spectroscopy
Boi Hanh Huynh
Part IV: Chemical Methods
16. Protocols for Cofactor Isoation of Nitrogenase
Aaron W. Fay, Chi-Chung Lee, Jared A. Wiig, Yilin Hu, and Markus W. Ribbe
17. Techniques for Functional and Structural Modeling of Nitrogenase
Patrick L. Holland
Part V: Theoretical Calculations
18. Nitrogenase Structure and Function Relationships by Density Functional Theory
Travis V. Harris and Robert K. Szilagyi
19. Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory
Gregory M. Sandala and Louis Noodleman
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