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Multiprotein Complexes: Methods and Protocols

Multiprotein Complexes: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 2247

BiologyChemistry

ISBN10: 1071611283
ISBN13: 9781071611289
Publisher: Humana
Published: Dec 12 2021
Pages: 340
Weight: 1.35
Height: 0.74 Width: 7.00 Depth: 10.00
Language: English
This volume explores strategies and detailed protocols for the preparation of macromolecular complexes and their characterization in view of structural analysis. The chapters in this book are separated into three parts: Part One focuses on sample preparation, and covers strategies for recombinant expression of multiprotein complexes in prokaryotic and eukaryotic hosts, for genome engineering using the CRISPR/Cas9 system and for production of specific binders such as reformatted antibodies and artificial binding proteins. Part Two looks at the biophysical methods that can provide useful indicators for sample optimization, and often complement structural information obtained with core technologies for structure determination--x-ray crystallography and cryo-electron microscopy--by quantitative solution data. Part Three discusses the characterization of multiprotein complexes in a cellular environment using the latest technologies and in vivo approaches. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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