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Microbial Steroids: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1645
Please select the version of book you would like to purchase.
ISBN10: 1493984101
ISBN13: 9781493984107
Publisher: Humana Pr
Published: Aug 8 2018
Pages: 373
Weight: 1.19
Height: 0.80 Width: 6.14 Depth: 9.21
Language: English
ISBN13: 9781493984107
Publisher: Humana Pr
Published: Aug 8 2018
Pages: 373
Weight: 1.19
Height: 0.80 Width: 6.14 Depth: 9.21
Language: English
Preface...
Table of Contents...
Contributing Authors...
Marina V. Donova 2. Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins
Ignacio Herráiz 3. Genome Sequencing of Steroid Producing Bacteria with Illumina Technology
Victoria Y. Shtratnikova, Mikhail I. Schelkunov, and marina V. Donova 4. Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome
Alberto Sola-Landa, Antonio Rodríguez-García, Carlos Barreiro, and Rosario Pérez-Redondo 5. RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics
Antonio Rodríguez-García, Alberto Sola-Landa, and Carlos Barreiro 6. Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria
Carlos Barreiro, Alejandro Morales, Inés Vázquez-Iglesias, and Alberto Sola-Landa 7. Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805
Jessica K. Loraine and Margaret C. M. Smith 8. Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas
José M. Luengo and Elias R. Olivera 9. Selection of Biodegrading Phytosterol Strains
María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal 10. Stigmasterol Removal by an Aerobic Treatment System
Soledad Chamorro, Claudia Xavier, Víctor Hernández, José Becerra, and Gladys Vidal 11. Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione
Rodrigo A. Mancilla, Roberto Pavez-Díaz, and Andrea Muñoz-Orrego 12. Β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates
Marco P.C. Marques and Pedro Fernandez 13. Bioconversion of Phytosterols into Androstenedione by Mycobacterium
Kjell D. Josefsen, Anna Nordborg, and Håvard Sletta 14. Scale-Up of Phytosterols Bioconversion into Androstenedione
Sonia Martínez-Cámara, Esther Bahíllo, José-Luis Barredo, and Marta Rodríguez-Sáiz 15. Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331
Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García, and Beatriz Galán 16. Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD)
Surya Prakash and Abhay Bajaj 17. Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants
Sabrina Hoebenreich, Martin Spinck, and Nathalie Nett 18. Obtaining of 11α-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols
Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina Donova 19. Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus
Lidia Ortega-de los Ríos, José M. Luengo, and José M. Fernández-Cañón 20. Biotransformation of DHEA into 7α, 15α-diOH-DHEA
Hui Li, Jin Sun, and Zhenghong Xu 21. Stabilization of Enzymes by Using Thermophiles
Ana-Luisa Ribeiro, Mercedes Sánchez, Aurelio Hidalgo, and José Berenguer
Fabian B. Thygs and Juliane Merz 24. Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC)
Govinda Guevara, Julián Perera, and Juana-María Navarro-Llorens
Alberto Sola-Landa, Antonio Rodríguez-García, Carlos Barreiro, and Rosario Pérez-Redondo 5. RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics
Antonio Rodríguez-García, Alberto Sola-Landa, and Carlos Barreiro 6. Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria
Carlos Barreiro, Alejandro Morales, Inés Vázquez-Iglesias, and Alberto Sola-Landa 7. Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805
Jessica K. Loraine and Margaret C. M. Smith 8. Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas
José M. Luengo and Elias R. Olivera 9. Selection of Biodegrading Phytosterol Strains
María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal 10. Stigmasterol Removal by an Aerobic Treatment System
Soledad Chamorro, Claudia Xavier, Víctor Hernández, José Becerra, and Gladys Vidal 11. Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione
Rodrigo A. Mancilla, Roberto Pavez-Díaz, and Andrea Muñoz-Orrego 12. Β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates
Marco P.C. Marques and Pedro Fernandez 13. Bioconversion of Phytosterols into Androstenedione by Mycobacterium
Kjell D. Josefsen, Anna Nordborg, and Håvard Sletta 14. Scale-Up of Phytosterols Bioconversion into Androstenedione
Sonia Martínez-Cámara, Esther Bahíllo, José-Luis Barredo, and Marta Rodríguez-Sáiz 15. Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331
Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García, and Beatriz Galán 16. Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD)
Surya Prakash and Abhay Bajaj 17. Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants
Sabrina Hoebenreich, Martin Spinck, and Nathalie Nett 18. Obtaining of 11α-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols
Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina Donova 19. Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus
Lidia Ortega-de los Ríos, José M. Luengo, and José M. Fernández-Cañón 20. Biotransformation of DHEA into 7α, 15α-diOH-DHEA
Hui Li, Jin Sun, and Zhenghong Xu 21. Stabilization of Enzymes by Using Thermophiles
Ana-Luisa Ribeiro, Mercedes Sánchez, Aurelio Hidalgo, and José Berenguer
22. Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media
Carla C. C. R. de Carvalho and Pedro Fernandes
Fabian B. Thygs and Juliane Merz 24. Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC)
Govinda Guevara, Julián Perera, and Juana-María Navarro-Llorens
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