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Metalloproteins: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1876
ISBN13: 9781493988631
Publisher: Humana
Published: Oct 13 2018
Pages: 275
Weight: 1.58
Height: 0.69 Width: 7.00 Depth: 10.00
Language: English
Part I. Metalloproteins
1. Nitrogenases
Nate Sickerman, Yilin Hu, and Markus W. Ribbe
2. Bacteriochlorophyll and Coenzyme F430
Jürgen Moser and Gunhild Layer
3. Carbon Monoxide Dehydrogenases
Jae-Hun Jeoung, Berta M. Martins, and Holger Dobbek
4. Molybdenum-Containing Enzymes
Dimitri Niks and Russ Hille
5. Hydrogenases
Nathaniel S. Sickerman and Yilin Hu
Part II. Genetic and Biochemical Methods
6. Genomic Manipulations of the Diazotroph Azotobacter vinelandii
Patricia Dos-Santos
7. Purification of Nitrogenase Proteins
Chi Chung Lee, Markus W. Ribbe, and Yilin Hu
8. Expression, Purification and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a, c-Diamide Reductase
Jürgen Moser, Jan Jasper, José Vazquez Ramos, Sven T. Sowa, and Gunhild Layer
9. Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactor
Paul Kaufmann, Chantal Iobbi-Nivol, and Silke Leimkühler
Part III. Structural and Spectroscopic Methods
10. Crystallization of Nitrogenase Proteins
Belinda B. Wenke, Renee J. Arias, and Thomas Spatzal
11. X-Ray Crystallography of Carbon Monoxide Dehydrogenases
Jae-Hun Jeoung, Berta M. Martins, and Holger Dobbek
12. X-Ray Absorption Spectroscopy of Metalloproteins
Limei Zhang
13. Electron Paramagnetic Resonance Spectroscopy of Metalloproteins
Andrew Jasniewski, Yilin Hu, and Markus Ribbe
14. Magnetic Circular Dichroism Spectroscopy of Metalloproteins
Brian J. Hales
Part IV. Chemical methods and Theoretical calculations
15. Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site
Kazuki Tanifuji and Yasuhiro Ohki
16. Computational Methods for Modeling Metalloproteins
Martin Stiebritz and Yilin Hu
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