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Lipid-Activated Nuclear Receptors: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1951
ISBN13: 9781493991297
Publisher: Humana
Published: Mar 4 2019
Pages: 220
Weight: 1.38
Height: 0.56 Width: 7.00 Depth: 10.00
Language: English
1. Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS
Lilia Magomedova and Carolyn L. Cummins
2. A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands
Samantha A. Hutchinson and James L. Thorne
3. Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages
Jean-Baptiste Julla, Raphaelle Ballaire, Tina Ejlalmanesh, Jean-François Gautier, Nicolas Venteclef, and Fawaz Alzaid
4. Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages
Emelie Barreby, André Sulen, and Myriam Aouadi
5. Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity
Laura Alonso-Herranz, Jesús Porcuna, and Mercedes Ricote
6. Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells
Ana Ramón-Vázquez, Juan Vladimir de la Rosa, Carlos Tabraue, and Antonio Castrillo
7. Dual Crosslinking Chromatin-Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages
David A. Rollins and Inez Rogatsky
8. Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics
Juan Vladimir de la Rosa, Ana Ramón-Vázquez, Carlos Tabraue, and Antonio Castrillo
9. Quantifying Cellular Cholesterol Efflux
Sabrina Robichaud and Mireille Ouimet
10. Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection
Estibaliz GlarÃa, Jonathan Matalonga, and Annabel F. Valledor
11. Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency
Matthew C. Gage
12. Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution
Ada Weinstock and Edward A. Fisher
13. Preparation of Frozen Liver Tissues for Integrated Omics Analysis
Ning Liang, Rongrong Fan, Saioa Goni, and Eckardt Treuter
14. Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver
Natalia Becares and Inés Pineda-Torra
15. The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo
Yasmine Sebti, Lise Ferri, Mathilde Zecchin, Justine Beauchamp, Denis Mogilenko, Bart Staels, Hélène Duez, and Benoit Pourcet
16. Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry
Kirsty E. Waddington, Inés Pineda-Torra
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