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Light Microscopy: Methods and Protocols

Light Microscopy: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 689

BiologyGeneral Science

ISBN10: 1607619490
ISBN13: 9781607619499
Publisher: Springer Nature
Published: Dec 15 2010
Pages: 244
Weight: 1.41
Height: 0.80 Width: 7.20 Depth: 10.20
Language: English
TABLE OF CONTENTS: Table of Contents Preface Contributors Part I. Bright-field Microscopy Applications 1. Glycol methacrylate embedding for improved morphological, morphometrical and immunohistochemical investigations under light microscopy: testes as a model Hélio Chiarini-Garcia, Gleydes Gambogi Parreira and Fernanda Raddichi Almeida 2. Histological processing of teeth and periodontal tissues for light microscopy analysis. Gerluza A. Borges Silva, Adriana Moreira and José Bento Alves 3. Large plant samples: how to process for 2-hydroxyethyl methacrylate embedding? Élder Antônio Sousa Paiva, Sheila Zambello de Pinho and Denise Maria Trombert Oliveira 4. Image cytometry: nuclear and chromosomal DNA quantification Carlos Roberto Carvalho, Wellington Ronildo Clarindo and Isabella Santiago de Abreu 5. Histological approaches to study tissue parasitism during Trypanosoma cruzi infection Daniela L. Fabrino, Lívia Teixeira, Grazielle Ribeiro and Rossana C.N. Melo 6. Intravital microscopy to study leukocyte recruitment in vivo Vanessa Pinho, Fernanda Matos Coelho, Gustavo Batista Menezes and Denise Carmona Cara Part II. Fluorescence Microscopy Applications 7. Introduction to fluorescence microscopy Ionita Ghiran 8. Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals Ernani Amaral, Silvia Guatimosim and Cristina Guatimosim 9. Imaging lipid bodies within leukocytes with different light microscopy techniques Rossana C. N. Melo, Heloísa D Ávila, Patrícia T. Bozza and Peter F. Weller 10. EicosaCell - An immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites. Christianne Bandeira-Melo, Peter F. Weller and Patricia T. Bozza 11. Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer Robert M. Hoffman 12. Imaging calcium sparks in cardiac myocytes Silvia Guatimosim, Cristina Guatimosim and Long-Sheng Song 13. Light microscopy in aquatic ecology: methods for plankton communities studies Maria Carolina S. Soares, Lúcia M. Lobão, Luciana O. Vidal, Natália P. Noyma, Nathan O. Barros, Fábio Roland 14. Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedding samples. Shin-ichi Iwasaki and Hidekazu Aoyagi

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