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Uno studio diagnostico comparativo sullo Streptococcus pneumoniae
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ISBN10: 6209966225
ISBN13: 9786209966224
Publisher: Edizioni Sapienza
Published: Jun 29 2026
Pages: 104
Weight: 0.33
Height: 0.25 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786209966224
Publisher: Edizioni Sapienza
Published: Jun 29 2026
Pages: 104
Weight: 0.33
Height: 0.25 Width: 6.00 Depth: 9.00
Language: Italian
Nel periodo compreso tra aprile 2010 e maggio 2011 sono stati prelevati trecento e tre campioni di liquido cerebrospinale presso sei ospedali iracheni da pazienti (bambini di età fino a 5 anni) con sospetta infezione da meningite microbica; tali campioni sono stati successivamente inviati al Laboratorio Centrale di Sanità Pubblica di Baghdad. La diagnosi di meningite batterica è stata presa in considerazione quando il quadro clinico e l'esame obiettivo lo suggerivano, con particolare attenzione allo Streptococcus pneumoniae in quanto importante agente patogeno responsabile della meningite pneumococcica nei bambini. I campioni di liquido cerebrospinale sono stati trasferiti in terreno Trans Isolate al Laboratorio Centrale di Sanità Pubblica di Baghdad, quindi messi in coltura su agar sangue e agar cioccolato. Sono stati eseguiti metodi di identificazione convenzionali, tra cui: (test di sensibilità all'optochina, test di solubilità nella bile, colorazione di Gram, emolisi del sangue umano, API 20 Strep, test di agglutinazione al lattice). L'identificazione molecolare è stata effettuata mediante estrazione del DNA dallo Streptococcus pneumoniae con diversi metodi, seguita dalla PCR.
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