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Intracellular Lipid Transport: Methods and Protocols

Intracellular Lipid Transport: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1949

BiologyChemistry

ISBN10: 1493991353
ISBN13: 9781493991358
Publisher: Humana Pr
Published: Feb 21 2019
Pages: 345
Weight: 1.85
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English

This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or at membrane contact sites, or within cellular membranes. The volume begins with methodologies to measure the movement of varied lipid species between or in organelle membranes, inside eukaryotic cells, including plant cells, or in bacteria, and continues in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoints, how lipid transfer proteins (LTPs) or flippases/scramblases precisely function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Chemistry