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Interaction of Proteins with Spherical Polyelectrolyte Brushes

Interaction of Proteins with Spherical Polyelectrolyte Brushes

Paperback

Chemistry

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ISBN10: 3832528660
ISBN13: 9783832528669
Publisher: Logos Verlag Berlin
Published: Jun 7 2011
Pages: 151
Weight: 0.40
Height: 0.33 Width: 5.68 Depth: 8.04
Language: English
Spherical polyelectrolyte brushes are a novel class of carrier particles for the
immobilization of proteins. These particles consist of a polystyrene core onto which
long chains of polyelectrolytes are grafted. A high uptake of proteins can be
achieved if the ionic strength is low, while both compounds carry an overall
negative charge. No adsorption takes place at higher ionic strength. This thesis
provides a deeper insight in the adsorption process of proteins onto polyelectrolyte
brushes. Here, the kinetics of the adsorption process is investigated by the
combination of time resolved small angle X-ray scattering and rapid stopped flow
mixing. The adsorption kinetics was described experimentally and theoretically.
Furthermore, the thermodynamics of the adsorption process was investigated by
isothermal titration calorimetry. The combination of experimental finding and
theoretical considerations identify the counterion release force as the main driving
force for the adsorption. Counterions from the brush layer and the protein surface
are released at low ionic strength and the free energy of the system will be
decreased. Furthermore, the behavior of the proteins in the adsorbed state was
investigated by the combination of several analytical techniques. These provide an
in-depth understanding of the kinetic activity, the protein structure and the
location of the adsorbed protein molecules in the polyelectrolyte layer.

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