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Handbook of Cryo-Preparation Methods for Electron Microscopy
Hardcover
Series: Methods in Visualization
ISBN13: 9780849372278
Publisher: Crc Pr Inc
Published: Oct 2 2008
Pages: 706
Weight: 3.00
Height: 1.40 Width: 7.00 Depth: 10.00
Language: English
Documenting the exponential growth in cryo-electron microscopy, the resulting advances, new hypotheses, and heightened understanding of the ultrastructure of living and inert matter, this book employes an accessible, step-by-step format to present the most sophisticated methods used in research laboratories today. It describes the basic principles of cryo-fixation, cryo-ultramicrotomy, embedding at low temperatures, and freeze-fracture. It explores applications in electron crystallography, 2D/3D structure analysis, cryo-electron tomography, and techniques for protein localization. Each chapter covers a specific method, including detailed protocols, summary tables, and hundreds of illustrations and diagrams.
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