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Heterologous Protein Production in Cho Cells: Methods and Protocols

Heterologous Protein Production in Cho Cells: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 1603

BiologyChemistry

ISBN10: 1493983563
ISBN13: 9781493983568
Publisher: Humana
Published: Jul 20 2018
Pages: 252
Weight: 1.02
Height: 0.55 Width: 7.00 Depth: 10.00
Language: English

This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using 'omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.

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