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Glycosyltransferases: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1022
ISBN13: 9781493959969
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 425
Weight: 1.70
Height: 0.91 Width: 7.00 Depth: 10.00
Language: English
1. Glycan Microarray Screening Assay for Glycosyltransferase Specificities
Wenjie Peng, Corwin M. Nycholat, and Nahid Razi
2. A Fluorescence-Based Assay for Core 1 3Galactosyltransferase (T-synthase) Activity
Tongzhong Ju and Richard D. Cummings
3. Structural and Biochemical Analysis of a Bacterial Glycosyltransferase
Fan Zhu, Ren Wu, Hua Zhang, and Hui Wu
4. Study of the Biological Functions of Mucin Type Core 3 O-glycans
Seung Ho Lee and Minoru Fukuda
5. Generation of Anti-Sulfated Glycan Antibodies using Sulfotransferase-deficient Mice
Hiroto Kawashima
6. Fluorescent Microscopy as a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in COG Complex Depleted Mammalian Cells
Rose A. Willett, Irina D. Pokrovskaya, and Vladimir V. Lupashin
7. A Practical Approach to Reconstruct Evolutionary History of Animal Sialyltransferases and Gain Insights into the Sequence-Function Relationships of Golgi-Glycosyltransferases
Daniel Petit, Roxana Elin Teppa, Jean-Michel Petit, and Anne Harduin-Lepers
8. Fluorescent Lectin Staining of Drosophila Embryos and Tissues to Detect the Spatial Distribution of Glycans during Development
E. Tian, Liping Zhang, and Kelly G. Ten Hagen
9. Photoaffinity Labeling of Protein O-Mannosyltransferases of the PMT1/PMT2 Subfamily
Martin Loibl and Sabine Strahl
10. Enzymatic Analysis of the Protein O-glycosyltransferase, Rumi, Acting toward Epidermal Growth Factor-like (EGF) Repeats
Hideyuki Takeuchi and Robert S. Haltiwanger
11. Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling
Eun Ju Kim and John Hanover
12. Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase, and Assay of its Substrate, UDP-GlcNAc
Tony Lefebvre, Ludivine Drougat, Stéphanie Olivier-van Stichelen, Jean-Claude Michalski, and Anne-Sophie Vercoutter-Edouart
13. In vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases
Matias A. Musumeci, Maria V. Ielmini, and Mario F. Feldman
14. In vitro UDP-sugar: Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection by Thin Layer Chromatography
Kinnari B. Patel and Miguel A. Valvano
15. In vitro O-Antigen Ligase Assay
Xiang Ruan and Miguel A. Valvano
16. Functional Identification of Bacterial Glucosyltransferase WbdN
Yin Gao, Anna Vinnikova, and Inka Brockhausen
17. Methods for the Pasteurella Glycosaminoglycan Synthases: Enzymes that Polymerize Hyaluronan, Chondroitin, or Heparosan Chains
Paul DeAngelis
18. Methods for Measuring Class I Membrane-bound Hyaluronan Synthase Activity
Paul H. Weigel, Amy J. Padgett-McCue, and Bruce A. Baggenstoss
19. Creation and Characterization of Glycosyltransferase Mutants of Trypanosoma brucei
Luis Izquierdo, M. Lucia S. Güther, and Michael A.J. Ferguson
20. Mannose-6-Phosphate: A Regulator of LLO Destruction
Ningguo Gao and Mark A. Lehrman
21. N-Acetylglucosaminyltransferase (GnT) Assays using Fluorescent Oligosaccharide Acceptor Substrates: GnT-III, IV, V, and IX (GnT-Vb)
Shinji Takamatsu, Hiroaki Korekane, Kazuaki Ohtsubo, Suguru Oguri, Jong Yi Park, Akio Matsumoto, and Naoyuki Taniguchi
22. A Method for Determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI Activity
Tomohiko Taguchi and Naoyuki Taniguchi
23. In vitro Assays of Orphan Glycosyltransferases and their Application to Identify Notch Xylosyltransferases
Maya K. Sethi, Falk F
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