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Gel-Free Proteomics: Methods and Protocols

Gel-Free Proteomics: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 753

BiologyChemistryGeneral Science

ISBN10: 1617791474
ISBN13: 9781617791475
Publisher: Humana
Published: May 30 2011
Pages: 400
Weight: 1.95
Height: 1.10 Width: 7.10 Depth: 10.10
Language: English

Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

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