Sign-In
Cart
Fundamentals of Fluorescence Microscopy: Exploring Life with Light
Paperback
Technology & EngineeringBiologyGeneral Science
Please select the version of book you would like to purchase.
ISBN10: 940177966X
ISBN13: 9789401779661
Publisher: Springer Nature
Published: Aug 23 2016
Pages: 218
Weight: 1.19
Height: 0.50 Width: 8.25 Depth: 11.00
Language: English
ISBN13: 9789401779661
Publisher: Springer Nature
Published: Aug 23 2016
Pages: 218
Weight: 1.19
Height: 0.50 Width: 8.25 Depth: 11.00
Language: English
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Also in
General Science
The Salmon Cannon and the Levitating Frog: And Other Serious Discoveries of Silly Science
York, Carly Anne
Hardcover
$27.00
The Code Breaker: Jennifer Doudna, Gene Editing, and the Future of the Human Race
Isaacson, Walter
Hardcover
$12.99
Ancient Astrology in Theory and Practice: A Manual of Traditional Techniques, Volume I: Assessing Planetary Condition
George, Demetra
Paperback
$61.25
The Quantum Power of GLP-1 Peptides: Unlocking the Science that Leads to Lasting Weight Loss and Optimal Healthspan
Seeds, William A.
Paperback
$34.20
The Reference Manual of Woody Plant Propagation: From Seed to Tissue Culture, Second Edition
Heuser Jr, Charles W.
Dirr, Michael A.
Paperback
$24.99
Stay Curious and Keep Exploring: 50 Amazing, Bubbly, and Creative Science Experiments to Do with the Whole Family
Calandrelli, Emily
Hardcover
$23.70
A History of the World in Six Plagues: How Contagion, Class, and Captivity Shaped Us, from Cholera to Covid-19
Bonhomme, Edna
Hardcover
$26.99
Outer Space Is Closer Than Antarctica: And Other Things I Learned While Falling in Love at the Bottom of the World
Ott, Michelle
Hardcover
$17.96
Pseudoscience: An Amusing History of Crackpot Ideas and Why We Love Them
Kang, Lydia
Pedersen, Nate
Hardcover
$22.50
Baked to Perfection: Winner of the Fortnum & Mason Food and Drink Awards 2022
Cermelj, Katarina
Hardcover
$27.00
Notes on Complexity: A Scientific Theory of Connection, Consciousness, and Being
Theise, Neil
Paperback
$18.00
They Had Names: Tracing the History of the North American Indigenous People
Jeanson, Nathaniel
Hardcover
$20.89
Judas Dentistry: How Dentists Scorn Science, Break the Hippocratic Oath, and Wreck Their Patients' Minds and Bodies
Yoho, (Ret) Robert
Paperback
$23.75
Rad Future: The Untold Story of Nuclear Electricity and How It Will Save the World
Boemeke, Isabelle
Hardcover
$28.50
5 Steps to a 5: AP Biology 2026: Practice AP Tests + AP Content Review + Digital Exams
Burris, Kelcey
Anestis, Mark
Paperback
$27.85
Designing for People: An Introduction to Human Factors Engineering
Boyle, Linda Ng
Wickens, Christopher D.
Liu, Yili
Paperback
$93.60
Alive: Synthetic Cells, Feral Robots, Rebellious Ai, and the Design of Radical Life
Schwartzman, Madeline
Hardcover
$47.50
Labwork to Leadership: A Concise Guide to Thriving in the Science Job You Weren't Trained for
Heemstra, Jen
Hardcover
$47.94
The Ups and Downs of Physics: The Science of Gravity, Aerodynamics, and Everyday Mishaps
Weber, Judith
Weber, Marcus
Hardcover
$28.45
Stuff Matters: Exploring the Marvelous Materials That Shape Our Man-Made World
Miodownik, Mark
Paperback
$17.09
The Problem of Increasing Human Energy: With Special Reference to the Harnessing of the Sun's Energy
Tesla, Nikola
Paperback
$7.53
Life in Sync: The Science of Internal Clocks and How We're Disrupting Them
Gander, Philippa
Hardcover
$21.80
You Look Like a Thing and I Love You: How Artificial Intelligence Works and Why It's Making the World a Weirder Place
Shane, Janelle
Paperback
$18.99
Counterclockwise: Using Peptides to Renew, Rejuvenate, and Rediscover (Large Print Edition)
Ferree, Suzanne J.
Hardcover
$28.03
The Forgotten Sense: The New Science of Smell--And the Extraordinary Power of the Nose
Olofsson, Jonas
Hardcover
$26.60
The Pale Blue Data Point: An Earth-Based Perspective on the Search for Alien Life
Willis, Jon
Hardcover
$24.70
The Theoretical Minimum: What You Need to Know to Start Doing Physics
Susskind, Leonard
Hrabovsky, George
Paperback
$18.04
Is This an Agate?: An Illustrated Guide to Lake Superior's Beach Stones Michigan
Robinson, Susan
Paperback
$7.55
Allies for Awakening: Guidelines for productive and safe experiences with entheogens
Metzner, Ralph
Paperback
$19.00
True Hallucinations: Being an Account of the Author's Extraordinary Adventures in the Devil's Paradis
McKenna, Terence
Paperback
$17.09
Elementary Science Education: Building Foundations of Scientific Understanding, Vol. II, grades 3-5, 2nd ed.
Nebel, Bernard J.
Paperback
$56.19
The Physics of Wall Street: A Brief History of Predicting the Unpredictable
Weatherall, James Owen
Paperback
$18.99
Science and Cooking: Physics Meets Food, from Homemade to Haute Cuisine
Weitz, David
Brenner, Michael
Sörensen, Pia
Hardcover
$33.25
AI The Magic Box: An Activity Book on Artificial Intelligence
Chung, Peggy
Seay, Danielle
Banjarian, Rani
Paperback
$23.99
Science Fictions: How Fraud, Bias, Negligence, and Hype Undermine the Search for Truth
Ritchie, Stuart
Paperback
$23.74
Middle School Science Education: Building Foundations of Scientific Understanding, Vol. III, Grades 6-8
Nebel, Bernard J.
Paperback
$44.95
Unreliable: Bias, Fraud, and the Reproducibility Crisis in Biomedical Research
Szabo, Csaba
Paperback
$45.00
Liquid Rules: The Delightful and Dangerous Substances That Flow Through Our Lives
Miodownik, Mark
Paperback
$15.19
The Peer Specialist's pocket resource for mental health and substance use services second edition
Drebing, Charles
Paperback
$18.05
Radical Abundance: How a Revolution in Nanotechnology Will Change Civilization
Drexler, K. Eric
Hardcover
$39.00
The College Student's Research Companion: Finding, Evaluating, and Citing the Resources You Need to Succeed, Sixth Edition
Rodda Quaratiello, Arlene
Paperback
$37.49
Reactions: An Illustrated Exploration of Elements, Molecules, and Change in the Universe
Gray, Theodore
Paperback
$18.99
We Are Electric: Inside the 200-Year Hunt for Our Body's Bioelectric Code, and What the Future Holds
Adee, Sally
Paperback
$18.99
The Scientist's Guide to Writing, 2nd Edition: How to Write More Easily and Effectively Throughout Your Scientific Career
Heard, Stephen B.
Paperback
$34.94
The Peptide Protocols: Exploring the Science for Human Optimization: Exploring the
Smith, W. T.
Paperback
$23.74
Mini Weapons of Mass Destruction: Build Implements of Spitball Warfare: Volume 1
Austin, John
Paperback
$17.09
Observer's Sky Atlas: The 500 Best Deep-Sky Objects with Charts and Images
Karkoschka, Erich
Hardcover
$37.95
T-Minus AI: Humanity's Countdown to Artificial Intelligence and the New Pursuit of Global Power
Kanaan, Michael
Hardcover
$26.55
Sé Más Feliz (You Happier Spanish Edition): Los 7 Secretos de la Neurociencia Para Sentirte Bien Según Tu Tipo de Cerebro.
G. Amen, Daniel G.
Paperback
$18.95
Hunt for the Skinwalker: Science Confronts the Unexplained at a Remote Ranch in Utah
Kelleher, Colm A.
Knapp, George
Paperback
$18.04
180 Days(tm) Geography for Fourth Grade: Practice, Assess, Diagnose
Aracich, Charles
Aracich, Chuck
Paperback
$21.84
Lab 257: The Disturbing Story of the Government's Secret Germ Laboratory
Carroll, Michael C.
Paperback
$18.04
A Very Convenient Warming: How Modest Warming and More Co2 Are Benefiting Humanity
Wrightstone, Gregory
Paperback
$18.95