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Fluorescent Proteins: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2564
ISBN13: 9781071626665
Publisher: Humana
Published: Sep 16 2022
Pages: 326
Weight: 1.78
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
1. Using FPbase: The Fluorescent Protein Database
Talley J. Lambert
2. Selecting the Fluorescent Protein for In Vivo Imaging Experiments
Mayank Sharma
3. Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo
Marcela DÃaz and Leonel Malacrida
4. Directed Evolution of Fluorescent Proteins in Bacteria
Sara Mattson, Geraldine N. Tran, and Erik A. Rodriguez
5. Fluorescent Proteins: Crystallization, Structural Determination, and Non-Natural Amino Acid Incorporation
Rochelle D. Ahmed, Husam Sabah Auhim, Harley L. Worthy, and D. Dafydd Jones
6. Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization
Alina Remeeva, Anna Yudenko, Vera V. Nazarenko, Oleg Semenov, Anastasia Smolentseva, Andrey Bogorodskiy, Ivan Maslov, Valentin Borshchevskiy, and Ivan Gushchin
7. Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization
Nora Lisa Bitzenhofer, Fabienne Hilgers, Gabriela N. Bosio, Joaquim Torra, Giorgia Casini, Felix R.M. Beinlich, Esther Knieps-Grünhagen, Valentin Gordeliy, Karl-Erich Jaeger, Santi Nonell, Ulrich Krauss, Thomas Gensch, and Thomas Drepper
8. CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11 Tag into the Genome of a Human Cell Line
Ryo Tamura and Daichi Kamiyama
9. Fluorescent Protein-Based Approaches for Sub-Cellular Protein Localization in Plants
Mayank Sharma, Ralf Bernd Klösgen, and Bationa Bennewitz
10. Growth Rates Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions
Kara L. Schneider, Lucas E. Reibenspies, Thomas Nyström, and Sviatlana Shashkova
11. DNA Visualization Using Fluorescent Proteins
Xuelin Jin, Y. Tehee Kim, and Kyubong Jo
12. Quantification of Intracellular Citrate Concentrations with Genetically Encoded Biosensors
Yufeng Zhao, Kei Takahashi-Yamashiro, Yi Shen, and Robert E. Campbell
13. Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out
Finja Bohle, Andreas J. Meyer, and Stefanie J. Mueller-Schuessele
14. Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae
Mikel Lavilla-Puerta and Beatrice Giuntoli
15. Osmium Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells
Isei Tanida, Junji Yamaguchi, Soichiro Kakuta, a
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