Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
612-822-4611

  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Fermentative production of recombinant haloalkane dehalogenases

Fermentative production of recombinant haloalkane dehalogenases

Polinek, Jiri

Paperback

Chemistry

Currently unavailable to order

ISBN10: 3838365399
ISBN13: 9783838365398
Publisher: Lap Lambert Academic Pub
Published: May 23 2010
Pages: 76
Weight: 0.27
Height: 0.18 Width: 6.00 Depth: 9.00
Language: English
The study was focused on fermentative production of haloalkane dehalogenases (HLDs), bacterial enzymes with ability to hydrolytically cleave carbon-halogen bond in aliphatic hydrocarbons. Fermentative production of three HLDs, LinBwt, DhaA31 and DatAwt, was established and compared to yields from the shake flask cultivations, the yields were similar (LinBwt and DhaA31) or higher (DatAwt). Two important factors affecting formation of biomass and protein yield were identified and investigated: (i) composition and (ii) growth supporting ability of a cultivation medium. The investigations revealed that nutritional value of yeast extract and glucose content in the cultivation medium has significant impact on effectiveness of the cultivations. Scaled-up fermentative production of HLDs is essential step for application in bioremediation of environmental pollutants, synthesis of fine chemicals, construction of biosensors, decontamination of warfare agents and cell imaging.

Also in

Chemistry

View All