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Fe-S Proteins: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2353
ISBN13: 9781071616079
Publisher: Humana
Published: Jul 24 2022
Pages: 351
Weight: 1.39
Height: 0.75 Width: 7.00 Depth: 10.00
Language: English
Part I - Methods for genetic manipulation and Fe-S protein expression
1. Molecular biology and genetic tools to investigate functional redundancy among Fe-S cluster carriers in E. coli
Yohann Duverger and Béatrice Py
2. Study Fe-S cluster proteins in Methanococcus maripaludis, a model archaeal organism
Cuiping Zhao, Christina A Roberts, Ian J Drake, and Yuchen Liu
3. Genetic approaches to uncover gene products involved in iron-sulfur protein maturation: High throughput genomic screening using transposon-sequencing
Valerie J. Carabetta, Karla Esquilin-Lebron, Ehud Zelzion, Jeffrey M. Boyd
4. Methods for heterologous overproduction of Fe-S proteins
Elliot I. Corless and Edwin Antony
5. Enzymatic and Chemical in vitro Reconstitution of Iron-Sulfur Cluster Proteins
Mauro Marengo, Rita Puglisi, Simonetta Oliaro-Bosso, Annalisa Pastore and Salvatore Adinolfi
6. Expression, isolation, and characterization of vanadium nitrogenase from Azotobacter vinelandii
Katharina Grunau, Jakob Gies-Elterlein, Christian Trncik and Oliver Einsle
Part II - Biochemical methods to probe steps involved in the synthesis of Fe-S clusters
7.Ni-NTA affinity chromatography to characterize protein-protein interactions during Fe-S cluster biogenesis
Terrell D. Carter and F. Wayne Outten
8. tRNA modifications as a readout of S and Fe-S metabolism
Ashley M. Edwards, Maame A. Addo, Patricia C. Dos Santos
9. Methods to unravel the roles of ATPases in Fe-S cluster biosynthesis
Christa N. Molé, Kinjal Dave, Deborah L. Perlstein
10. Methods to investigate the kinetic profile of cysteine desulfurases
Maame A. Addo, Ashley M. Edwards, Patricia C. Dos Santos
11. A fast and ratiometric method for quantification of cysteine-bound persulfides based on alkylation and gel-shift assays
Sylvain Gervason, Batoul Srour, and Benoit D'Autréaux
Part III - Biophysical methods to study Fe-S clusters and reactions promoted by metalloenzymes
12. Circular dichroism to probe the synthesis, transfer, and stability of Fe-S clusters
Stefania Iametti, Francesco Bonomi, and Alberto Barbiroli
13. Native mass spectrometry of iron-sulfur proteins
Jason C. Crack, and Nick E. Le Brun
14. Characterization of Paramagnetic Iron-Sulfur Clusters using Electron Paramagnetic Resonance Spectroscopy
Troy A. Stich
15. Characterization of Fe-S clusters in proteins by Mӧssbauer Spectroscopy
Chie Ueda, Michelle Langton, and Maria-Eirini Pandelia
16. Biochemical approaches to probe the role of the auxiliary iron-sulfur cluster of lipoyl synthase from Mycobacterium tuberculosis
Vivian Robert Jeyachandran, Jay V. Pendyala, Erin L. McCarthy, Amie K. Boal, and Squire J. Booker
17. Methods to Screen for Radical SAM Enzyme Crystallization Conditions
Lydie Martin, Xavier Vernède, and Yvain Nicolet
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