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Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 955
ISBN13: 9781493958764
Publisher: Humana
Published: Aug 23 2016
Pages: 586
Weight: 2.27
Height: 1.22 Width: 7.00 Depth: 10.00
Language: English
1. Introduction to Electron Crystallography
Werner Kühlbrandt
2. Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis
Kutti R. Vinothkumar, Patricia C. Edwards, and Joerg Standfuss
3. Two-Dimensional Crystallization of Membrane Proteins by Reconstitution through Dialysis
Matthew C. Johnson, Tina M. Dreaden, Laura Y. Kim, Frederik Rudolph, Bridgette Barry, and Ingeborg Schmidt-Krey
4. Monolayer Two-Dimensional Crystallization
Luc Lebeau and Catherine Vénien-Bryan
5. Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples
Tina M. Dreaden, Maureen Metcalfe, Laura Y. Kim, Matthew C. Johnson, Bridgette A. Barry, and Ingeborg Schmidt-Krey
6. Low Dose Techniques and Cryo-Electron Microscopy
Yoshinori Fujiyoshi
7. Grid Preparation for Cryo-Electron Microscopy
Nobuhiko Gyobu
8. Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins
Agustin Avila-Sakar, Xueming Li, Shawn Q. Zheng, and Yifan Cheng
9. The Collection of High-Resolution Electron Diffraction Data
Tamir Gonen
10. Image Processing of 2D Crystal Images
Marcel Arheit, Daniel Castaño-Diéz, Raphael Thierry, Bryant R. Gipson, Xiangyan Zeng, and Henning Stahlberg
11. Merging of Image Data in Electron Crystallography
Marcel Arheit, Daniel Castaño-Diéz, Raphael Thierry, Priyanka Abeyrathne, Bryant R. Gipson, and Henning Stahlberg
12. Evaluation of Electron Crystallographic Data From Images of Two-Dimensional Crystals
Vinzenz M. Unger
13. Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy
Gregory S. Allen and David L. Stokes
14. Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement
Goragot Wisedchaisri and Tamir Gonen
15. High-Throughput Methods for Electron Crystallography
David L. Stokes, Iban Ubarretxena-Belandia, Tamir Gonen, and Andreas Engel
16. Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening
Anchi Cheng
17. Automation of Data Acquisition in Electron Crystallography
Anchi Cheng
18. Automation of Image Processing in Electron Crystallography
Marcel Arheit, Daniel Castaño-Diéz, Raphael Thierry, Bryant R. Gipson, Xiangyan Zeng, and Henning Stahlberg
19. Choice and Maintenance of Equipment for Electron Crystallography
Deryck J. Mills and Janet Vonck
20. Future Developments in Instrumentation for Electron Crystallography
Kenneth H. Downing
21. Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction
Peijun Zhang, Xin Meng, and Gongpu Zhao
22. Single Particle Electron Microscopy
Wilson C.Y. Lau and John L. Rubinstein
23. Electron Tomography of Paracrystalline 2D Arrays
Hanspeter Winkler, Shenping Wu, and Kenneth A. Taylor
24. High-Resolution Imaging of 2D Outer Membrane Protein F (OmpF) Crystals by Atomic Fo
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