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Erk Signaling: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1487
Please select the version of book you would like to purchase.
ISBN10: 1493964224
ISBN13: 9781493964222
Publisher: Humana Pr
Published: Dec 6 2016
Pages: 440
Weight: 2.20
Height: 1.00 Width: 7.00 Depth: 10.00
Language: English
ISBN13: 9781493964222
Publisher: Humana Pr
Published: Dec 6 2016
Pages: 440
Weight: 2.20
Height: 1.00 Width: 7.00 Depth: 10.00
Language: English
1. How Genetics has Helped Piece Together the MAPK Signaling Pathway
Dariel Ashton-Beaucage and Marc Therrien
2. In Vitro Enzyme Kinetics Analysis of EGFR
Zhihong Wang and Christine Candelora
3. High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells
Nobuo Yoshimoto and Shun'ichi Kuroda 4. Structural Studies of ERK2 Protein Complexes
Johannes F. Weijman, Stefan J. Riedl, and Peter D. Mace 5. Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk
Tal Goshen-Lago, Dganit Melamed, Arie Admon, and David Engelberg 6. Assaying Activation and Subcellular Localization of ERK in Cells and Tissues
Carme Caelles, Carles Bayod, and Melisa Morcillo 7. Detection and Functional Analysis of SUMO-Modified MEK
Yuji Kubota and Mutsuhiro Takekawa 8. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag
Liu Yang and Alexey Veraksa 9. High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates
Rona Grossman and Ze'ev Paroush 10. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE
Hidetaka Kosako and Kou Motani 11. Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation
Lorena Agudo-Ibañez, Piero Crespo, and Berta Casar 12. Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions
Raffaele Strippoli, Asier Echarri, Miguel Angel del Pozo 13. The Nuclear Translocation of ERK
Denise A. Berti and Rony Seger 14. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA)
Zijian Tang and Chengkai Dai 15. Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors
Yannick Blum, Rafael D. Fritz, Hyunryul Ryu, and Olivier Pertz 16. Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers
Hiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, and Masahiro Sokabe 17. Co-Culture Activation of MAP Kinase in Drosophila S2 Cells
Josefa Steinhauer 18. Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling
Marc K. Saba-El-Leil, Christophe Frémin, and Sylvain Meloche 19. 3D Organotypic Culture Model to Study Components of ERK Signaling
Athina-Myrto Chioni, Rabia Tayba Bajwa, and Richard Grose 20. Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway
Carmen G. Lechuga, LucÃa Simón-Carrasco, Harrys K.C. Jacob, and Matthias Drosten 21. Genome-Wide Analysis of RAS/ERK Signaling Targets
Joshua P. Plotnik and Peter C. Hollenhorst 22. Probing Chromatin Modifications in Response to ERK Signaling
Ozgur Oksuz and Wee-Wei Tee 23. Analyzing pERK Activation during Planarian Regeneration
Susanna Fraguas, Yoshihiko Umesono, Kiyokazu Agata, and Francesc CebriÃ
24. Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development
Jessica Jie Chen and Swathi Arur 25. Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images
Bomyi Lim, Carmeline J. Dsilva, Ioannis G. Kevrekidis, and Stanislav Y. Shvartsman
Johannes F. Weijman, Stefan J. Riedl, and Peter D. Mace 5. Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk
Tal Goshen-Lago, Dganit Melamed, Arie Admon, and David Engelberg 6. Assaying Activation and Subcellular Localization of ERK in Cells and Tissues
Carme Caelles, Carles Bayod, and Melisa Morcillo 7. Detection and Functional Analysis of SUMO-Modified MEK
Yuji Kubota and Mutsuhiro Takekawa 8. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag
Liu Yang and Alexey Veraksa 9. High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates
Rona Grossman and Ze'ev Paroush 10. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE
Hidetaka Kosako and Kou Motani 11. Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation
Lorena Agudo-Ibañez, Piero Crespo, and Berta Casar 12. Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions
Raffaele Strippoli, Asier Echarri, Miguel Angel del Pozo 13. The Nuclear Translocation of ERK
Denise A. Berti and Rony Seger 14. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA)
Zijian Tang and Chengkai Dai 15. Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors
Yannick Blum, Rafael D. Fritz, Hyunryul Ryu, and Olivier Pertz 16. Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers
Hiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, and Masahiro Sokabe 17. Co-Culture Activation of MAP Kinase in Drosophila S2 Cells
Josefa Steinhauer 18. Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling
Marc K. Saba-El-Leil, Christophe Frémin, and Sylvain Meloche 19. 3D Organotypic Culture Model to Study Components of ERK Signaling
Athina-Myrto Chioni, Rabia Tayba Bajwa, and Richard Grose 20. Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway
Carmen G. Lechuga, LucÃa Simón-Carrasco, Harrys K.C. Jacob, and Matthias Drosten 21. Genome-Wide Analysis of RAS/ERK Signaling Targets
Joshua P. Plotnik and Peter C. Hollenhorst 22. Probing Chromatin Modifications in Response to ERK Signaling
Ozgur Oksuz and Wee-Wei Tee 23. Analyzing pERK Activation during Planarian Regeneration
Susanna Fraguas, Yoshihiko Umesono, Kiyokazu Agata, and Francesc CebriÃ
24. Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development
Jessica Jie Chen and Swathi Arur 25. Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images
Bomyi Lim, Carmeline J. Dsilva, Ioannis G. Kevrekidis, and Stanislav Y. Shvartsman
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