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E. Coli Plasmid Vectors: Methods and Applications

E. Coli Plasmid Vectors: Methods and Applications

Hardcover

Series: Methods in Molecular Biology, Book 235

Medical ReferenceChemistry

ISBN10: 1588291510
ISBN13: 9781588291516
Publisher: Springer Nature
Published: Jul 3 2003
Pages: 316
Weight: 1.41
Height: 0.88 Width: 6.20 Depth: 9.30
Language: English
A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

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