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Drosophila Cytogenetics Protocols

Drosophila Cytogenetics Protocols

Hardcover

Series: Methods in Molecular Biology, Book 247

Medical ReferenceGeneral MathematicsZoology

ISBN10: 1588290506
ISBN13: 9781588290502
Publisher: Springer Nature
Published: Nov 4 2003
Pages: 468
Weight: 1.99
Height: 1.08 Width: 6.28 Depth: 9.12
Language: English
Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

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Zoology