Sign-In
Cart
DNA Cloning and Assembly Methods
Hardcover
Series: Methods in Molecular Biology, Book 1116
Medical ReferenceOther Animals
ISBN13: 9781627037631
Publisher: Springer Nature
Published: Jan 7 2014
Pages: 303
Weight: 1.69
Height: 0.75 Width: 7.00 Depth: 10.00
Language: English
In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.
With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.
1 different editions
Also available
Also in
Medical Reference
The Body Keeps the Score: Brain, Mind, and Body in the Healing of Trauma
Van Der Kolk, Bessel
Paperback
Overcoming Acute and Chronic Pain: Keys to Treatment Based on Your Emotional Type
Dibra, Sebhia Marie
Micozzi, Marc S.
Paperback
Working The Roots: Over 400 Years of Traditional African American Healing
Lee, Michele Elizabeth
Paperback
The New Menopause: Navigating Your Path Through Hormonal Change with Purpose, Power, and Facts
Haver, Mary Claire
Hardcover
Jesus & Your Mental Health: Linking God's Word and Modern Science to Find Peace about Mental Health
Maxwell Lmft, Rebecca
Paperback
How to Forage for Mushrooms Without Dying: An Absolute Beginner's Guide to Identifying 29 Wild, Edible Mushrooms
Hyman, Frank
Paperback
Forbidden Facts: Government Deceit & Suppression about Brain Damage from Childhood Vaccines
de Becker, Gavin
Hardcover
Change Your Brain, Change Your Pain: Breaking the Doom Loop to Heal Chronic Physical and Emotional Pain
Amen, Daniel G.
Hardcover
The Big One: How We Must Prepare for Future Deadly Pandemics
Osterholm, Michael T.
Olshaker, Mark
Hardcover
Science Under Siege: How to Fight the Five Most Powerful Forces That Threaten Our World
Mann, Michael E.
Hotez, Peter J.
Hardcover
Food Intelligence: The Science of How Food Both Nourishes and Harms Us
Belluz, Julia
Hall, Kevin
Hardcover
Anatomy Coloring Book with 450+ Realistic Medical Illustrations with Quizzes for Each
McCann, Stephanie
Wise, Eric
Paperback
Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (Dsm-5-Tr(r))
American Psychiatric Association
Paperback
Period Repair Manual: Natural Treatment for Better Hormones and Better Periods
Briden Nd, Lara
Paperback
The Mindful Self-Compassion Workbook: A Proven Way to Accept Yourself, Build Inner Strength, and Thrive
Neff, Kristin
Germer, Christopher
Paperback
The Dysautonomia Project: Understanding Autonomic Nervous System Disorders for Physicians and Patients
Goldstein, Phd
Thompson, Charles R.
Freeman, Msm Kelly
Paperback
The Complete Native American Healing Herbs Encyclopedia - 7 Books in 1
Garcia Navarro, Hialeah
Paperback
Longevity Guidebook: How to Slow, Stop, and Reverse Aging - and NOT Die from Something Stupid
Diamandis, Peter H.
Hardcover
The 36-Hour Day: A Family Guide to Caring for People with Alzheimer Disease, Other Dementias, and Memory Loss
Rabins, Peter V.
Mace, Nancy L.
Paperback
El Cuerpo Lleva La Cuenta: Cerebro, Mente Y Cuerpo En La Superación del Trauma / The Body Keeps the Score
Van Der Kolk, Bessel
Paperback
The Ultimate Survival Medicine Guide: Emergency Preparedness for Any Disaster
Alton, Amy
Alton, Joseph
Paperback
Destiny of the Republic: A Tale of Madness, Medicine and the Murder of a President
Millard, Candice
Paperback
Why We Get Sick: The Hidden Epidemic at the Root of Most Chronic Disease--And How to Fight It
Bikman, Benjamin
Paperback
What Happened to You?: Conversations on Trauma, Resilience, and Healing
Perry, Bruce D.
Winfrey, Oprah
Hardcover
Shoulder Pain? The Solution & Prevention: Fifth Edition, Revised & Expanded
Kirsch, John M.
Paperback
Murder by Injection: The Story of the Medical Conspiracy Against America
Mullins, Eustace Clarence
Paperback
All Creatures Great and Small: The Warm and Joyful Memoirs of the World's Most Beloved Animal Doctor
Herriot, James
Paperback
The Gut-Brain Paradox: Improve Your Mood, Clear Brain Fog, and Reverse Disease by Healing Your Microbiome
Gundry MD, Steven R.
Hardcover
Everything Is Tuberculosis: The History and Persistence of Our Deadliest Infection (Large Print Edition)
Green, John
Paperback
Plant Powered Plus: Activate the Power of Your Gut to Tame Inflammation and Reclaim Your Health
Bulsiewicz, Will
Hardcover
Integrating a Palliative Approach Workbook 2nd Edition, Revised: Essentials For Personal Support workers
Murray, Katherine
Paperback
How to Make Your Brain Your Best Friend: A Neuroscientist's Guide to a Healthier, Happier Life
Barr, Rachel
Hardcover
Medical Apartheid: The Dark History of Medical Experimentation on Black Americans from Colonial Times to the Present
Washington, Harriet A.
Paperback
The Perimenopause Survival Guide: Make Sense of Your Symptoms and Build Your Personalized Treatment Plan
Hirsch, Heather
Hardcover
The Heart Code: The Breakthrough Method to Open Your Heart and Transform Your Life
Nelson, Bradley
Nelson, Jean
Hardcover
How to Change Your Mind: What the New Science of Psychedelics Teaches Us about Consciousness, Dying, Addiction, Depression, and Transcendence
Pollan, Michael
Paperback
Other Minds: The Octopus, the Sea, and the Deep Origins of Consciousness
Godfrey-Smith, Peter
Paperback
Taking Charge of Adult ADHD: Proven Strategies to Succeed at Work, at Home, and in Relationships
Barkley, Russell A.
Paperback
The Metabolic Approach to Cancer: Integrating Deep Nutrition, the Ketogenic Diet, and Nontoxic Bio-Individualized Therapies
Winters, Nasha
Kelley, Jess Higgins
Hardcover
Dissolving Illusions: Disease, Vaccines, and The Forgotten History
Bystrianyk, Roman
Humphries, Suzanne
Paperback
God's Hotel: A Doctor, a Hospital, and a Pilgrimage to the Heart of Medicine
Sweet, Victoria
Paperback
THE DYSAUTONOMIA PROJECT Second Edition
Freeman, Kelly
Goldstein, David S.
Thompson, Charles R.
Paperback
Desk Reference to the Diagnostic Criteria from Dsm-5-Tr(r)
American Psychiatric Association
Paperback
Sensory Pathways to Healing from Trauma: Harnessing the Brain's Capacity for Change
Lanius, Ruth A.
Harricharan, Sherain
Kearney, Breanne E.
Paperback
Child Medication Fact Book for Psychiatric Practice, Second Edition
Feder, Joshua D.
Puzantian, Talia
Tien, Elizabeth
Paperback
Ob/GYN Peds Notes: Nurse's Clinical Pocket Guide
Kratovil, Amanda
Holloway, Brenda Walters
Moredich, Cheryl
Paperback
The Ivf Blueprint: Everything You Need to Know about in Vitro Fertilization, Egg Freezing, and Embryo Transfer
Hudson, Susan
Eblen, Abby
Bedient, Carrie
Hardcover
Unwinding Bone: A Complete Guide to Biodynamic Skeletal Therapy for Myofascial and Manual Therapists
Sternthal, Scott
Paperback
The Period Brain: The New Science of Why We PMS and How to Fix It: A Manifesto for Women
Hill Ph. D., Sarah E.
Hardcover
Estrogen Matters: Why Taking Hormones in Menopause Can Improve and Lengthen Women's Lives -- Without Raising the Risk of Breast Cancer (2024 Revised a
Tavris, Carol
Bluming, Avrum
Hardcover
The Good Death: A Guide for Supporting Your Loved One Through the End of Life
O'Brien, Suzanne B.
Hardcover
The Formula for Better Health: How to Save Millions of Lives--Including Your Own
Frieden, Tom
Hardcover
Trauma and Recovery: The Aftermath of Violence--From Domestic Abuse to Political Terror
Herman, Judith Lewis
Paperback
Nursing School Entrance Exams Prep: Your All-In-One Guide to the Kaplan and Hesi Exams
Kaplan Nursing
Paperback
The Great Shadow: A History of How Sickness Shapes What We Do, Think, Believe, and Buy
Bauer, Susan Wise
Hardcover
The Boy Who Was Raised as a Dog: And Other Stories from a Child Psychiatrist's Notebook -- What Traumatized Children Can Teach Us about Loss, Love, an
Perry, Bruce D.
Szalavitz, Maia
Paperback
The Quantum Power of GLP-1 Peptides: Unlocking the Science that Leads to Lasting Weight Loss and Optimal Healthspan
Seeds, William A.
Paperback
The Devil's Castle: Nazi Eugenics, Euthanasia, and How Psychiatry's Troubled History Reverberates Today
Antonetta, Susanne Paola
Hardcover
Clinical Reasoning: Coaching the Struggling Medical Learner
Parsons, Andrew S.
Warburton, Karen M.
Paperback
Gut and Psychology Syndrome: Natural Treatment for Autism, Dyspraxia, A.D.D., Dyslexia, A.D.H.D., Depression, Schizophrenia, 2nd Edition
Campbell-McBride, Natasha
Paperback
The End of Alzheimer's Program: The First Protocol to Enhance Cognition and Reverse Decline at Any Age
Bredesen, Dale
Paperback
Witches, Midwives, & Nurses (Second Edition): A History of Women Healers
Ehrenreich, Barbara
English, Deirdre
Paperback
The Addictive Personality: Understanding the Addictive Process and Compulsive Behavior
Nakken, Craig
Paperback
Dissolving Illusions: Disease, Vaccines, and the Forgotten History 10th Anniversary Edition
Humphries, Suzanne
Bystrianyk, Roman
Paperback
The Anti-Inflammatory Diet Slow Cooker Cookbook: Prep-And-Go Recipes for Long-Term Healing
Given, Madeline
Paperback
College Mental Health 101: A Guide for Students, Parents, and Professionals
Willard, Christopher
Aguirre, Blaise
Green, Chelsie
Paperback
Hidden Epidemic: Silent Oral Infections Cause Most Heart Attacks and Breast Cancers
Levy, Jd
Paperback
The Poisoner's Handbook: Murder and the Birth of Forensic Medicine in Jazz Age New York
Blum, Deborah
Paperback
Women in White Coats: How the First Women Doctors Changed the World of Medicine
Campbell, Olivia
Paperback
If Sleep Were A Drug: The Science of Sleep Optimization: Biohack Your Brain, Body, and Performance
Grossman, Lt Col Dave
Kaylor, Leah
Paperback
The Age of Diagnosis: How Our Obsession with Medical Labels Is Making Us Sicker
O'Sullivan, Suzanne
Hardcover
Nursing Teas 7: A Quickstudy Laminated Reference Guide
Davis, April Michelle
Henry, Julie
Herrin, Jennifer
Other
Pcos SOS: A Gynecologist's Lifeline To Naturally Restore Your Rhythms, Hormones, and Happiness
Gersh, Felice
Perella, Alexis
Paperback
EMT Exam Prep, Eighth Edition: Focused Prep for the Nremt Cognitive Exam + One Full Length Practice Test
Kaplan Medical
Paperback
Herbal Antibiotics and Antivirals: Herbal Medicine to Heal Yourself Naturally
Samons, Brittany
Paperback
Stem Cell Therapy: A Rising Tide: How Stem Cells Are Disrupting Medicine and Transforming Lives
Riordan, Neil H.
Paperback
Another World Is Possible: Lessons for America from Around the Globe
Hakimi Zapata, Natasha
Hardcover
Nclex-RN Prep, Twenty-Sixth Edition (2025): Includes 1 Full Length Practice Test + Proven Strategies
Kaplan Nursing
Paperback
Pro-Life: Saving the Lives of Unborn Children, Making Possible Their Descendants, and Helping Their Parents
Harrison, Jim
Paperback
The Ghost Map: The Story of London's Most Terrifying Epidemic--And How It Changed Science, Cities, and the Modern World
Johnson, Steven
Paperback
Cure Your Fatigue, Second Edition: The Root Cause and How to Fix It on Your Own
Robbins, Morley M.
Paperback
Invisible No More: Embracing Your Road to Recovery from Long Covid and Other Complex Chronic Illnesses
Ruhoy, Ilene Sue
Hardcover
Good Moms Have Scary Thoughts: A Healing Guide to the Secret Fears of New Mothers
Kleiman, Karen
Hardcover
Desk Reference to the Diagnostic Criteria from Dsm-5-Tr(r)
American Psychiatric Association
Paperback
Clean -- Expanded Edition: The Revolutionary Program to Restore the Body's Natural Ability to Heal Itself
Junger, Alejandro
Paperback
Mastering Diabetes: The Revolutionary Method to Reverse Insulin Resistance Permanently in Type 1, Type 1.5, Type 2, Prediabetes, and Gestational Diabe
Barbaro, Robby
Khambatta, Cyrus
Paperback
Accessing the Healing Power of the Vagus Nerve: Self-Help Exercises for Anxiety, Depression, Trauma, and Autism
Rosenberg, Stanley
Paperback
The Friendship Bench: How Fourteen Grandmothers Inspired a Mental Health Revolution
Chibanda, Dixon
Paperback
The American Plague: The Untold Story of Yellow Fever, The Epidemic That Shaped Our History
Crosby, Molly Caldwell
Paperback
Cancer Hates Tea: A Unique Preventive and Transformative Lifestyle Change to Help Crush Cancer
Uspenski, Maria
Paperback
Dying of Whiteness: How the Politics of Racial Resentment Is Killing America's Heartland
Metzl, Jonathan M.
Paperback
The Vaccine-Friendly Plan: Dr. Paul's Safe and Effective Approach to Immunity and Health-From Pregnancy Through Your Child's Teen Years
Thomas, Paul
Margulis, Jennifer
Paperback
Cocktail Codex: Fundamentals, Formulas, Evolutions [A Cocktail Recipe Book]
Kaplan, David
Fauchald, Nick
Day, Alex
Hardcover
Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (Dsm-5-Tr(r))
American Psychiatric Association
Hardcover
American Sirens: The Incredible Story of the Black Men Who Became America's First Paramedics
Hazzard, Kevin
Paperback
Anger Management Skills Workbook for Kids: 40 Awesome Activities to Help Children Calm Down, Cope, and Regain Control
Robinson, Amanda
Paperback
Infected: How Power, Politics, and Privilege Use Science Against the World's Most Vulnerable
Zaman, Muhammad H.
Hardcover
MCAT Complete 7-Book Subject Review 2026-2027, Set Includes Books, Online Prep, 3 Practice Tests
Kaplan Test Prep
Paperback
Saunders Comprehensive Review for the Nclex-Rn(r) Examination
Silvestri, Angela
Silvestri, Linda Anne
Paperback
After: A Doctor Explores What Near-Death Experiences Reveal about Life and Beyond
Greyson, Bruce
Paperback
Living Well with Adult ADHD: Practical Strategies for Improving Your Daily Life
Knouse, Laura E.
Barkley, Russell A.
Paperback
The Acid Alkaline Food Guide: A Quick Reference to Foods and Their Effect on PH Levels
Brown, Susan E.
Trivieri, Larry
Paperback
The Spirit Catches You and You Fall Down: A Hmong Child, Her American Doctors, and the Collision of Two Cultures
Fadiman, Anne
Paperback