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Collagen: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1944
ISBN13: 9781493990948
Publisher: Humana Pr
Published: Mar 7 2019
Pages: 254
Weight: 1.50
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
Part I: In Vitro Models for Collagen Characterization
1. Cloning and Mutagenesis Strategies for Large Collagens
Olivier Bornert and Alexander Nyström
2. Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen
Venkat Raghavan Krishnaswamy
3. Production and Characterization of Chemically Cross-Linked Collagen Scaffolds
Ignacio Sallent, Héctor Capella-MonsonÃs, and Dimitrios I. Zeugolis
4. A Biomimetic Model for Mineralization of Type-I Collagen Fibrils
Shasha Yao, Yifei Xu, Changyu Shao, Fabio Nudelman, Nico A.J.M. Sommerdijk, and Ruikang Tang
Part II: Proteomics Analysis of Collagen
5. Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy
Wing Ying Chow
6. Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry
Amar Joshi, Amna Zahoor, and Alberto Buson
7. Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes
Ngoc-Duc Doan, Andrew S. DiChiara, Amanda M. Del Rosario, Richard P. Schiavoni, and Matthew D. Shoulders
8. Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics
Elizabeta Madzharova, Fabio Sabino, and Ulrich auf dem Keller
Part III: Imaging Approaches for Collagen
9. Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy
Elee Shimshoni and Irit Sagi
10. In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology
Yang Li and S. Michael Yu
11. Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics
Stéphane Bancelin, Barbara Lynch, Christelle Bonod-Bidaud, Petr Dokládal, Florence Ruggiero, Jean-Marc Allain, and Marie-Claire Schanne-Klein
12. Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix
Uwe Hansen
Part IV: Collagen in Health and Disease
13. Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans
Alina C. Teuscher, Cyril Statzer, Sophia Pantasis, Mattia R. Bordoli, and Collin Y. Ewald
14. Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis
Efrat Kessler and Eyal Hassoun
15. Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment
Chen Varol
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