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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Capillary Electrophoresis: Methods and Potentials

Capillary Electrophoresis: Methods and Potentials

Engelhardt, Heinz
Gutnikov, G.
Beck, Wolfgang

Paperback

Medical ReferenceTechnology & EngineeringChemistry

ISBN10: 3642858562
ISBN13: 9783642858567
Publisher: Springer Nature
Published: Jun 12 2012
Pages: 215
Weight: 0.81
Height: 0.48 Width: 6.69 Depth: 9.61
Language: English
Capillary electrophoresis (CE), also designated by the acronym HPCE for High Per- formance Capillary Electrophoresis is a rapidly growing analytical separation method. It unites the separation technique of classical electrophoresis on plates with the instrumental methods of chromatography with respect to direct detection of the solutes separated in the capillary and their ready identification and quantification. The initial problems of inadequate reproducibility in quantitative analysis, due to the necessity of handling extremely small volumes, have largely been solved in the sec- ond generation commercial instruments. Hence, a rapid and reliable separation sys- tem is available for ionic compounds from the smallest cation (the lithium ion) up to poly anions with molecular weights ranging in the millions (such as DNA molecules). The methods of gel electrophoresis and isoelectric focusing can be readily extended to separation techniques carried out in a capillary. For nonionic compounds an addi- tional separation method is available in the form of micellar electrokinetic chroma- tography (MEKC). This involves a true chromatographic separation process because the distribution of the analytes between the buffer and the micelles is superimposed on the electrophoretic migration, which contributes substantially to the selectivity.

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