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Cytochrome P450 Protocols
Hardcover
Series: Methods in Molecular Biology, Book 987
ISBN13: 9781627033206
Publisher: Humana
Published: Mar 9 2013
Pages: 319
Weight: 1.55
Height: 0.90 Width: 7.10 Depth: 10.00
Language: English
1. Bioluminescent Assays for Cytochrome P450 Enzymes
Douglas S. Auld, Henrike Veith, and James J. Cali
2. In Vitro Drug-Drug Interaction Assay
Michael Zientek and Kuresh Youdim
3. High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening
Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel
4. The Synthesis, Characterization and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites
Christopher McCullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel Sem
5. High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators
Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg
6. Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches
Qian Cheng and F. Peter. Guengerich
7. Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis
Hugues Ouellet, Eric D. Chow, Shenheng Guan, Jeffery S. Cox, Alma L. Burlingame, and Paul R. Ortiz de Montellano
8. An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins
Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta
9. Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group
Wesley E. Straub, Clinton R. Nishida, and Paul R. Ortiz de Montellano
10. Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes
A. Luthra, M. Gregory, Y.V. Grinkova, I.G. Denisov, and S.G. Sligar
11. Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles
Linlin Zhao, Besnik Bajrami, and James F. Rusling
12. Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells
M. Teresa Donato and M. José Gómez-Lechón
13. Screening for Cytochrome P450 Reactivity with a Reporter Enzyme
Kersten S. Rabe and Christof M. Niemeyer
14. High-Throughput Fluorescence Assay of Cytochrome P450 3A4
Qian Cheng and F. Peter. Guengerich
15. Targeted Protein Capture for Analysis of Electrophile-Protein Adducts
Rebecca E. Connor, Simona G. Codreanu, Lawrence J. Marnett, and Daniel C. Liebler
16. DNA Shuffling of Cytochrome P450 Enzymes
James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam
17. Measurement of P450 Difference Spectra Using Intact Cells
Wayne A. Johnston and Elizabeth M.J. Gillam
18. DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production
Nedeljka N. Rosic
19. P450 oxidoreductase - Genotyping, Expression, Purification of Recombinant Protein and Activity Assessments of Wild-Type and Mutant Protein
Vishal Agrawal and Walter L. Miller
20. LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450
Federico Sabbadin, Gideon G. Grogan and Neil C. Bruce
21. Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-allele) Nomenclature Database
Sarah C. Sim and Magnus Ingelman-Sundberg
22. Simultaneous in vivo Phenotyping of CYP Enzymes
Sussan Ghassabian and Michael Murray
23. Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase-I Footprinting
Matthew Ha
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