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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Chromosome Combing

Chromosome Combing

Donatienne Ruby, Christabel

Paperback

Chemistry

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ISBN10: 6137769666
ISBN13: 9786137769669
Publisher: Fidel
Pages: 92
Weight: 0.32
Height: 0.22 Width: 5.98 Depth: 9.02
Language: English
Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Chromosome combing (also known as molecular combing or DNA combing) is a technique used to produce an array of uniformly stretched DNA that is then highly suitable for nucleic acid hybridization studies such as fluorescent in situ hybridisation (FISH) which benefit from the uniformity of stretching, the easy access to the hybridisation target sequences, and the resolution offered by the large distance between two probes, which is due to the stretching of the DNA by a factor of 1.5 times the crystallographic length of DNA.DNA in solution (i.e. has a random-coil structure) is stretched by retracting the meniscus of the solution at a constant rate (typically 300 m/s). The ends of DNA strands, which are thought to be frayed (i.e. open and exposing polar groups) bind to ionisable groups coating a silated glass plate at a pH below the pKa of the ionizable groups (ensuring they are charged enough to interact with the ends of DNA).

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