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Chloroplast Research in Arabidopsis: Methods and Protocols, Volume I
Paperback
Series: Methods in Molecular Biology, Book 774
BiologyBotany & HorticultureChemistry
ISBN13: 9781493958504
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 374
Weight: 1.47
Height: 0.80 Width: 7.00 Depth: 10.00
Language: English
Part I: Genetics, Cytology, and In Vivo Analysis
1. Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis
Enrique López-Juez and Alison Hills
2. Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy
Kevin Pyke
3. Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins
Stanislav Vitha and Katherine W. Osteryoung
4. Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts
Dong Wook Lee and Inhwan Hwang
5. Visualisation of Stromules on Arabidopsis Plastids
John C. Gray, James A. Sullivan, and Christine A. Newell
6. Analysis of Chloroplast Movement and Relocation in Arabidopsis
Masamitsu Wada and Sam-Geun Kong
7. Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots
John Stanga, Allison Strohm, and Patrick H. Masson
8. Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy
Stefan Hyman and R. Paul Jarvis
9. Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method
Kerry Ann Lutz, Arun Azhagiri, and Pal Maliga
Part II: Gene Expression and Protein Accumulation
10. Isolation, Quantification, and Analysis of Chloroplast DNA
Beth A. Rowan and Arnold J. Bendich
11. Measurement of Transcription Rates in Arabidopsis Chloroplasts
Yan O. Zubo, Thomas Börner, and Karsten Liere
12. Studying the Structure and Processing of Chloroplast Transcripts
Alice Barkan
13. In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts
Toshiharu Shikanai and Kenji Okuda
14. Studying Translation in Arabidopsis Chloroplasts
Paolo Pesaresi
15. Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts
Lars L.E. Sjögren and Adrian K. Clarke
Part III: Protein Transport, Localization, and Topology
16. In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins
Sandra Tanz and Ian Small
17. Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays
Henrik Aronsson and R. Paul Jarvis
18. Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions
Hitoshi Inoue, Fei Wang, Takehito Inaba, and Danny J. Schnell
19. Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays
Thomas Bals and Danja Schünemann
20. Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction
Chiung-Chih Chu and Hsou-min Li
21. Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases
John Froehlich
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