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Channelrhodopsin: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2191
ISBN13: 9781071608326
Publisher: Humana
Published: Sep 1 2021
Pages: 405
Weight: 1.60
Height: 0.86 Width: 7.00 Depth: 10.00
Language: English
Part I: Basic Science
1. Molecular Dynamics Simulations of Channelrhodopsin Chimera, C1C2
Monika R. VanGordon
2. Computing Potential of the Mean Force Profiles for Ion Permeation through Channelrhodopsin Chimera, C1C2
Chad Priest, Monika R. VanGordon, Caroline Rempe, Mangesh Chaudhari, Mark Stevens, Steve Rick, and Susan B. Rempe
3. Nanodisc Reconstitution of Channelrhodopsins Heterologously Expressed in Pichia pastoris for Biophysical Investigations
Maria Walter and Ramona Schlesinger
4. Characterizing Channelrhodopsin Channel Properties via Two-Electrode Voltage Clamp and Kinetic Modeling
Lindsey Prignano, Lauren Herchenroder, and Robert E. Dempski
Part II: Cells
5. Charge Transport by Light-Activated Rhodopsins Determined by Electrophysiological Recordings
Tamara Hussein and Christian Bamann
6. Probing Channelrhodopsin Electrical Activity in Algal Cell Populations
Oleg A. Sineshchekov, Elena G. Govorunova, and John L. Spudich
7. Two-Photon Optogenetic Stimulation of Drosophila Neurons
Mehmet Fişek and James M. Jeanne
8. Probing Synaptic Signaling with Optogenetic Stimulation and Genetically-Encoded Calcium Reporters
Gabriel B. Borja, Himali Shroff, Hansini Upadhyay, Pin W. Liu, Valeriya Baru, Yung-Chih Cheng, Owen B. McManus, Luis A. Williams, Graham T. Dempsey, and Christopher A. Werley
9. Optogenetics to Interrogate Neuron-Glia Interactions in Pups and Adults
Chloé Habermacher, Blandine Manot-Saillet, Domiziana Ortolani, Fernando C. Ortiz, and Maria Cecilia Angulo
10. Chronic Optogenetic Pacing of Human Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues
Marc Dwenger, William J. Kowalski, Hidetoshi Masumoto, Takeichiro Nakane, and Bradley B. Keller
Part III: Animals
11. Patterned Optogenetic Stimulation Using a DMD-Projector
Aanchal Bhatia, Sahil Moza, and Upinder S. Bhalla
12. Selecting Channelrhodopsin Constructs for Optimal Visual Restoration in Differing Light Conditions
Tushar H. Ganjawala and Zhuo-Hua Pan
13. Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish
Yagmur Idil Ozdemir, Christina A. Hansen, Mohamed A. Ramy, Eileen L. Troconis, Lauren D. McNeil, and Josef G. Trapani
14. Automated Functional Screening for Modulators of Optogenetically-Activated Neural Responses in Living Organisms
Ross C. Lagoy, Eric Larsen, and Dirk R. Albrecht
15. Optogenetic Interrogation of ChR2-Expressing GABAergic Interneurons
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