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Calcium Signaling Protocols
Paperback
Series: Methods in Molecular Biology, Book 937
ISBN13: 9781493959280
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 360
Weight: 1.43
Height: 0.77 Width: 7.00 Depth: 10.00
Language: English
Part I: General
1. Fluorescent Measurement of [Ca2]]c: Basic Practical Considerations
Alec W.M. Simpson
2. Measurement of [Ca2]]i in Whole Cell Suspensions Using Fura-2
Anish Patel, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert
Part II: Specialist Measurement Systems
3. Confocal Microscopy: Theory and Applications for Cellular Signaling
Stephen C. Tovey, Paul J. Brighton, Edward T.W. Bampton, Yan Huang, and Gary B. Willars
4. Ratiometric Ca2+ Measurements Using the FlexStation(R) Scanning Fluorometer
Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty
5. Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR(R))
Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty
6. Ratiometric [Ca2]]i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader
Benjamin D. Hunt and David G. Lambert
Part III: Measurement of Ca2+ Channel Activity
7. Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones
Jon Brown, Atticus H. Hainsworth, Alessandro Stefani, and Andrew D. Randall
8. Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells
Richard D. Rainbow
Part IV: Measurement of Ins(1,4,5)P3 and Ca2+ Release Form Intracellular Stores
9. Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells
Alison Skippen, Philip Swigart, and Shamshad Cockcroft
10. Single Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells
Carl P. Nelson
11. Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay
Darren Smart
Part V: Specialist Measurement Techniques
12. Measurement of [Ca2]]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
Hideo Kanaide and Katsuya Hirano
13. Calcium Measurements from Whole Heart Using Rhod-2
Bum-Rak Choi
14. Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries
Kim A. Dora and Michael A. Hill
15. Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration
John G. McCarron, Marnie L. Olson, Susan Chalmers, and John M. Girkin
16. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations
Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch
17. Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes
Denis Ottolini, Tito Calì, and Marisa Brini
18. Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors
Valeria Camarda and Girolamo Calo
19. Compartmentalizing Genetically-Encoded Calcium Sensors
David A. Williams, Mastura Monif, and Kate L. Richardson
20. Measurement of Cytosolic-Free Ca2+ in Plant Tissue
Martin R. McAinsh and Carl K.-Y. Ng
21. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1) Danuta Kosk-Kosicka
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