Argonaute Proteins: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 725
ChemistryGeneral ScienceGenetics
ISBN13: 9781617790454
Publisher: Springer Nature
Published: May 2 2011
Pages: 344
Weight: 1.80
Height: 1.00 Width: 7.00 Depth: 10.10
Language: English
1. Purification of Native Argonaute Complexes from the Fission Yeast Schizosaccharomyces pombe
Shane M. Buker and Mohammad R. Motamedi
2. Chromatin Immuno-Precipitation in Fission Yeast
Thomas A. Volpe and Jessica DeMaio
3. Biochemical Analyses of Endogenous Argonaute Complexes Immunopurified with Anti-Argonaute Monoclonal Antibodies
Keita Miyoshi, Tomoko N. Okada, Haruhiko Siomi, and Mikiko C. Siomi
4. Mapping of Ago2-GW182 Functional Interactions
Bing Yao, Songqing Li, Shangli Lian, Marvin J. Fritzler, and Edward K.L. Chan
5. Continuous Density Gradients to Study Argonaute and GW182 Complexes Associated with the Endocytic Pathway
Derrick Gibbings
6. In vitro RISC Cleavage Assay
Julia Stoehr and Gunter Meister
7. Native Gel Analysis for RISC Assembly
Tomoko Kawamata and Yukihide Tomari
8. Purification and Assembly of Human Argonaute, Dicer, and TRBP Complexes
Nabanita De and Ian J. MacRae
9. Detection of Human Dicer and Argonaute 2 Catalytic Activity
Marjorie P. Perron, Patricia Landry, Isabelle Plante, and Patrick Provost
10. Imaging the Cellular Dynamics of Drosophila Argonaute Proteins
Jing Li, Nima Najand, Wendy Long, and Andrew Simmonds
11. ----Live Cell Imaging of Argonaute Proteins in Mammalian Cells
Justin M. Pare, Joaquin Lopez-Orozco, and Tom C. Hobman
12. Reporter-Based Assays for Analyzing RNA Interference in Mammalian Cells
Lydia V. McClure, Gil Ju Seo, and Christopher S. Sullivan
13. Artificial Tethering of Argonaute Proteins for Studying their Role in Translational Repression of Target mRNAs
Stephanie Eckhardt, Emilia Szostak, Zhaolin Yang, and Ramesh Pillai
14. An Efficient System For Let-7 MicroRNA- And GW182 Protein-Mediated Deadenylation In vitro
Marc R. Fabian, Yuri V. Svitkin, and Nahum Sonenberg
15. Cell-Free microRNA-Mediated Translation Repression in C. elegans
Edlyn Wu and Thomas F. Duchaine
16. Argonaute Pull-Down and RISC Analysis Using 2'-O-Methylated Oligonucleotides Affinity Matrices
Guillaume Jannot, Alejandro Vasquez-Rifo, and Martin J. Simard
17. Cloning Argonaute-Associated Small RNAs from C. elegans
Weifeng Gu, Julie M. Claycomb, Pedro J Batista, Craig C. Mello, and Darryl Conte, Jr.
18. Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs
Yohei Kirino, Anastassios Vourekas, Eugene Khandros, and Zissimos Mourelatos
19. Generation of an Inducible Mouse ES Cell Line Deficient for Argonaute Proteins
Hong Su and Xiaozhong Wang
20. Whole Cell Proteome Regulation by microRNAs Captured in a Pulsed SILAC Mass Spectrometry Approach
Olivia Ebner and Matthias SelbachAlso in
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