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Adp-Ribosylation and Nad+ Utilizing Enzymes: Methods and Protocols

Adp-Ribosylation and Nad+ Utilizing Enzymes: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1813

Chemistry

ISBN10: 1493985876
ISBN13: 9781493985876
Publisher: Humana Pr
Published: Aug 11 2018
Pages: 417
Weight: 2.12
Height: 0.94 Width: 7.00 Depth: 10.00
Language: English

This volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Chemistry