Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
612-822-4611

  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Adaptive Wavefront Correction in Two-photon Microscopy - Coherence-Gated Wavefront Sensing

Adaptive Wavefront Correction in Two-photon Microscopy - Coherence-Gated Wavefront Sensing

Rückel, Markus

Paperback

Physics

ISBN10: 3639011554
ISBN13: 9783639011555
Publisher: Blues Kids Of Amer
Published: Apr 29 2008
Pages: 120
Weight: 0.38
Height: 0.25 Width: 6.00 Depth: 9.00
Language: English
The focus of a two-photon microscope is often degraded by inhomogeneities in the refractive index within biological specimens. In this book it is shown for various specimens, even for living zebrafish, that the resolution and the fluorescence signal of a two-photon microscope can be substantially improved by using adaptive optics, i.e. wavefront correction based on coherence-gated wavefront sensing (CGWS). The advantage of using CGWS relies on the fact that the wavefront distortions are sensed by backscattered instead of fluorescent light. Thus, neither photodamage nor photobleaching occurs and wavefront distortions can be sensed in less than 1 microsecond with an accuracy of lambda/50, even in strongly scattering samples. Furthermore, CGWS is thoroughly investigated for all relevant parameters affecting the measurement process, such as coherence length, polarization of the light, density of scatterers, and coherence-gate position. The book is mainly addressed to experts in the field of optical physics and light microscopy.

Also in

Physics

View All