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Apobec Enzymes: Volume 713

Apobec Enzymes: Volume 713

Hardcover

Series: Methods in Enzymology, Book 713

BiologyChemistry

ISBN10: 0443317860
ISBN13: 9780443317866
Publisher: Academic Press
Published: May 21 2025
Pages: 328
Weight: 1.44
Height: 0.69 Width: 6.39 Depth: 9.26
Language: English
Apobec Enzymes, Volume 713 in this series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Fluorescent shift assay for APOBECs RNA editing, Low Error Sequencing Methods to Detect APOBEC-mediated RNA editing: Circular RNAseq and Safe-Sequencing System, Safe-Barcode RNAseq assay for APOBECs RNA editing, DT40 cell system to characterize somatic hypermutation, CH12 cell system to assay AID activity on class switch recombination, Purification of Enzymatically Active APOBEC Proteins from an Insect Cell Expression System, and more.

Additional chapters cover Defining genome-wide mutagenic impact of APOBEC3 enzymes, APOBEC-induced mutational assay in yeast, Assays for APOBEC drug discovery, Biochemical assay for the identification of APOBECs inhibitors, An In Vitro Cytidine Deaminase Assay to Monitor APOBEC activity on DNA, Profiling rare C-to-U editing events via direct RNA sequencing, Global quantification of off-target activity by base editors, and so much more.

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Chemistry