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Analyzing Microbes: Manual of Molecular Biology Techniques
Hardcover
Series: Springer Protocols Handbooks
Medical ReferenceBiologyChemistry
ISBN13: 9783642344091
Publisher: Springer
Published: Feb 3 2013
Pages: 352
Weight: 1.78
Height: 1.05 Width: 7.23 Depth: 10.29
Language: English
Microbial DNA extraction, purification and quantitation.- Fluorescent based detection, quantitation and expression of wviral gene by qRT-PCR.- Restriction enzymes and their role in microbiology.- Genetic fingerprinting techniques for molecular characterization of microbes.- Agarose gel electrophoresis and Polyacrylamide agarose gel electrophoresis: Methods and principles.- Molecular identification of microbes.- Preservation and maintenance of microbial cultures.- Microbes from extreme environment: Molecular identification procedures.- ELISA based identification and detection of microbes.- Analysis of microbial diversity and construction of metagenomic library.- Bioinformatics tools for interpretation of data used in molecular identification.- Molecular phylogenetics of microbes.- Microarray technology: Basic concept, protocols and applications.- Microarray analysis of different functional genes of microorganisms.- DNA cloning and sequencing.- Biological sequence analysis: Algorithms and statistical methods.
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