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Amyloid Proteins: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 849
ISBN13: 9781617795503
Publisher: Humana
Published: Feb 24 2012
Pages: 548
Weight: 2.55
Height: 1.40 Width: 7.10 Depth: 10.10
Language: English
Part I. In Vivtro Models and Assays
1. Rapid Generation of Dityrosine Cross-linked Aβ Oligomers via Cu-redox Cycling
Adam P. Gunn, Blaine R. Roberts and Ashley I Bush
2. Applications of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins
Dahabada H.J. Lopes, Sharmisthia Sinha, Clark Rosenweig, Gal Bitan
3. Preparation of Stable Amyloid β-Protein Oligomers of Defined Assembly Order
Clark Rosenweig, Kenjiro One, Kazuma Murakami, Devin K. Lowenstein, Gal Bitan, David B. Teplow
4. Purifications and Fibrillation of Full-Length Recombinant PrP
Natallia Makarava and Ilia V. Baskakov
5. Featuring Amyloids with Fourier Transform Infrared and Circular Dichroism Spectroscopies
Miguel Calero and Maria Gasset
6. Quasielestic Light Scattering Study of Amyloid β-Protein Fibrillogenesis
Aleksey Lomakin and David B. Teplow
7. Conformations of Microtuble-Associated Protein Tau Mapped by Fluorescence Resonance Energy Transfer (FRET)
Sadasivam Jeganathan, Subashchandrabrose Chinnathamb, Eva-Maria Mandelkow, Echard Mandelkow
8. Measuring the Kinetics of Amyloid Fibril Elongation using Quartz Crystal Microbalances (QCM)
Alexander K. Buell, Christopher M. Dobson, and Mark E. Welland
9. X-ray Fibre Diffraction Studies of Amyloid Fibrils
Kyle L Morris and Louise C Serpell
10.Structural Characterization of Pre-fibrillar Intermediates and Amyloid Fibrils by Small-angle X-ray Scattering
Annette Eva Langkilde and Bente Vestergaard
11.Atomic Force Fluorescence Microscopy in the Characterization of Amyloid Fibril Assembly and Oligomeric Intermediates
12. Investigating Fibrillar Aggregates of Tau Protein by Atomic Force Microscopy
Susanne Wegmann, Daniel J. Muller and Eckhard Mandelkow
13. Structural Studies of Amyloids by Quenched Hydrogen-Deuterium Exchange by NMR
Marçal Vilar, Lei Wang and Roland Riek
14.Cyclic Amplification of Prion Protein Misfolding
Marcelo A Barria, Dennisse Gonzalez-Romero, and Claudio Soto
15. Search for Amyloid Binding Proteins by Affinity Chromatography
Miguel Calero, Agueda Rostagno, and Jorge Ghiso
Part II Cell Culture Models and Assays
16. Using Biophysical Approaches for Establishing the Links Between Aβ Aggregation and Cytotoxicity in Vitro
Asad Jan and Hilal A. Lashuel
17. Preparation of Cultured Human Vascular Cells
Ingvar H. Olafsson, Dadi Th. Vilhjalmsson and Finnbogi R. Thormodsson
18. Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells from Mouse Brain
Sebastien A. Gauthier, Susmita Sahoo, Sonia S. Jung, and Efrat Levy
19. In Vitro Assays Measuring Protection by Proteins such as Cystatin C of Primary Cortical Neuronal and Smooth Muscle Cells
Sebastein A. Gauthier, Belen Tizon, Susmita Sahoo, and Efrat Levy
20. Study of Neurotoxic Intracellular Calcium Signalling Triggered by Amyloids
Carlos Villalobos, Erica Caballero, Sara Sanz-Blasco and LucÃa Núñez
21. Bacterial Amyloids
Yizhou Zhou, Luz Blanco, Daniel R. Smith, Matthew R. Chapman
22. Study of Amyloids using Yeast
Reed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey and Herman K. Edskes
23. Cell-to-cell Transmission of a-synuclein Aggregate
Seung-Jae Lee, Paula Desplats, He-Jin Lee, Brian Spencer and Eliezer Masliah
Part III In Vivo Models and Assays
24. Subcutaneous Adipose Tissue Biopsy for Amyloid Protein Studies
Per Westermark
25. Analysis of S100 Oligomers and Amyloids
Hugo M. Botelho, Günter Fritz, Cláudio M. Gomes
26. S100A8/A9 Amyloidosis in the Ageing Prostate: Relating ex vivo and in vitro Studies
Anna L. Gharibyan, Dina Raveh, Ludmilla A.
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